掃一掃，添加微信號
|
Catalog # Product Description |
CN0885 This high affinity anti-GST-tag antibody is developed using the GST peptide conjugated to KLH. The peptide sequence (EEHLYERDEGDKWR) is derived from the common vector glutathione S transferase (protein code: ACH81576). |
|
Western blot analysis of cell expressing a GST fusion protein (45 kDa). Left lane: 5 μL of the negative cell lysates; right lane: 5 μL of the cell lysate, transfected with a vector coding GST-fusion protein.
|
|
Species
|
Rabbit
|
||
|
Formulation
|
250 μg/mL in PBS with 50% glycerol
|
||
|
Immunogen
|
GST peptide EEHLYERDEGDKWR KLH conjugates
|
||
|
Purification
|
Antigen-specific affinity chromatography
|
||
|
Specificity
|
The antibody recognizes the 37 kDa GADPH protein as a single band.
|
||
|
Applications
|
ELISA (1:2500); WB (1:500-2000); IF (5 μg/mL); IHC (5 μg/mL)
|
||
|
Scientific Description
|
GST is glutathione S transferase which is frequently used as an affinity tag in recombinant proteins to facilitate the isolation and localization of the target protein.
|
||
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is three years from date of shipping if properly stored.
|
